|
KMID : 0606920120200050487
|
|
Biomolecules & Therapeutics
2012 Volume.20 No. 5 p.487 ~ p.491
|
|
|
Effects of Rutaecarpine on Hydrogen Peroxide-Induced Apoptosis in Murine Hepa-1c1c7 Cells
|
|
Lee Sung-Jin
Ahn Hyun-jin
Nam Kung-Woo
Kim Kyeong-Ho
Mar Woong-Chon
|
|
|
Abstract
|
|
|
|
The aim of this study was to investigate the inhibitory effects of rutaecarpine on DNA strand breaks and apoptosis induced by hydrogen peroxide (H2O2) in murine Hepa-1c1c7 cells. Oxidative DNA damage was estimated by nuclear condensation assessment, fluorescence-activated cell sorting analysis, and Comet assay. Rutaecarpine inhibited cell death induced by 500 μM H2O2, as assessed by 4',6-diamidino-2-phenylindole (DAPI) staining. Treatment with rutaecarpine reduced the number of DNA strand breaks induced by H2O2, as assessed by DAPI staining and Comet assay, and increased quinone reductase, phosphatidylinositol 3-kinase, and pAkt protein levels, as assessed by western blotting.
|
|
|
KEYWORD
|
|
|
Rutaecarpine, Oxidative stress, DNA damage, Quinone reductase, Apoptosis, Hydrogen peroxide
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|